Staining bacterial polysaccharides.
نویسنده
چکیده
منابع مشابه
Quantification of polysaccharides fixed to Gram stained slides using lactophenol cotton blue and digital image processing
Dark blue rings and circles emerged when the non-specific polysaccharide stain lactophenol cotton blue was added to Gram stained slides. The dark blue staining is attributable to the presence of capsular polysaccharides and bacterial slime associated with clumps of Gram-negative bacteria. Since all bacterial cells are glycosylated and concentrate polysaccharides from the media, the majority of...
متن کاملQuantification of polysaccharides fixed to Gram stained slides using lactophenol cotton blue and digital image
Dark blue rings and circles emerged when the non-specific polysaccharide stain lactophenol cotton blue was added to Gram stained slides. The dark blue staining is attributable to the presence of capsular polysaccharides and bacterial slime associated with clumps of Gram-negative bacteria. Since all bacterial cells are glycosylated and concentrate polysaccharides from the media, the majority of...
متن کاملQuantification of polysaccharides fixed to Gram stained slides
I discovered indigo rings and circles in ATCC® 25922TM Escherichia coli cultures when I added the non-specific polysaccharide stain lactophenol cotton blue to Gram stained slides sampled from 96-well plates used to measure quantitative growth kinetics (QGK) in virtual colony count antimicrobial assays. I attribute the dark blue staining to the presence of capsular polysaccharides and bacterial ...
متن کاملLocation of bacterial polysaccharide during various phases of growth.
Polysaccharide-containing material was located by histochemical staining on ultrathin sections of Bacillus cereus and several clostridial species during various phases of growth. These components were located along the cell wall in young vegetative cells and along the developing cortex in sporulating cells. In B. cereus any carbohydrate-containing material present along the forespore membrane w...
متن کاملApplication of periodic acid-Schiff type stains to bacteria in milk.
The use of periodic acid oxidation followed by treatment with Schiff's reagent as a method for staining polysaccharides was first reported briefly by McManus (1946) and later in some detail by Hotchkiss (1948). Hotchkiss did not find the procedure particularly satisfactory for bacteria, but considered that it worked very well with many other polysaccharide materials. Despite the poor results re...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 66 4 شماره
صفحات -
تاریخ انتشار 1953